Mycelium Growing Liquid Culture Vials
Firstly, the culture/spores are boosted in liquid culture and, once growing, transferred to the rye grain. This leads to a more rapid colonisation and better yield in many of these species (however you could just inject a MycoFarm directly with P.tuberregium spores or culture but the results will not be as good and may even fail).
1 . Remove only the small centre circle from the aluminium cap leaving the rest of the cap in place. Swab the grey rubber centre portion and inject the liquid culture vial with approx 2.5mL of culture/spores. Incubate at correct temp (usually 20-25 C).
Mix contents by shaking gently every few days. Within 1-2 weeks, white fluffy growth of mycelium will be visible in the liquid. N.B. See photo: the liquid above the fluffy mycelia is still transparent if it is cloudy even after being left to settle for a few minutes it may be contaminated with bacteria. If so, best to get another vial and start again (contact your supplier).
2. This step needs to be done carefully, so get everything ready, read through first and have a practice run. Work in a clean area. Shake the vial vigorously, swab lid (keep swab handy for later) and turn vial upside down so the liquid is near the cap. Withdraw most of the liquid culture using a sterile syringe and leave a small amount remaining in the vial. It is not necessary to get every last drop (and trying to get the last this may increase risk of contaminants entering). Before removing the syringe/needle, grip the barrel/plunger carefully to stop it moving and the contents being sucked back into vial under pressure. Try not to draw any extra air into the syringe. The alcohol swab can be placed over needle when withdrawing to reduce risk of contamination.
3. Inoculate rye grain bag using 5-10mL of the culture